Type strains of Campylobacter 17 speciesArcobacter butleri and Helicobacter pylori, and 38 and 49 clinical isolates of C. To obtain biomass, all isolates were grown on Karmali agar Oxoid Inc. When primary and secondary primers were used together, PCR conditions remained the same except that 25 and 30 cycles were used in the primary and secondary reactions, respectively. For all PCR tests, positive controls consisted of genomic DNA from reference strains and negative controls consisted of water alone. To determine the sensitivity of detection, genomic DNA extracted from C. Primary and secondary PCRs as described above were performed. For C.
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